Similar to SASUKE, Last Woman Standing refers to the competitor, who does her run last in a KUNOICHI competition.
Miyake Ayako has the achieved the feat of Last Woman Standing in three competitions, the most in KUNOICHI history so far.
| Competition | Last Woman Standing | Result | Notes |
|---|---|---|---|
| 2001 | Yusa Masami | Second Stage: Daichoyaku | |
| 2002 | Watanabe Kazue | Final Stage: Tenkudou | About 2 meters across. |
| 2003 | Mizuno Yūko | Final Stage: Tenkubou | Reached the top of the tower in time, but pressed the button about 0.2 seconds late. |
| 2004 | Miyake Ayako | Final Stage: Kanzenseiha | Clear (4.11 seconds left). First woman to achieve Kanzenseiha in KUNOICHI. |
| 2006 New Year | Miyake Ayako | Final Stage: Kanzenseiha | Clear (7.74 seconds left). First woman to achieve Kanzenseiha twice in KUNOICHI. |
| 2006 Summer | Miyake Ayako | Final Stage: Kanzenseiha | Clear (0.66 seconds left). First woman to achieve Kanzenseiha three times in KUNOICHI. |
| 2007 | Tanaka Maho | Third Stage: Tourenban | Yellow Zone. |
| 2009 | Kadoi Satomi | Final Stage: Kanzenseiha | 21.44 seconds left. Third woman to obtain Kanzenseiha in KUNOICHI. |
| 2017 Spring | Izumi Hikari | Final Stage: Tsuna Nobori | About 13 metres up. Time Out. |
| 2017 Summer | Izumi Hikari | Third Stage: Pipe Slider | Failed the dismount. |
| 2018 | Okabe Sakiko | Final Stage: Tsuna Nobori | About 13.5 metres up. Time Out. |